Research on Biological Activity of Products Synthesized by Tetraketones and Arylidene-Arylamines

Based on the reaction of 1,6-diaryl-3, 4-dihydroxy-2, 4-hexadiene-1, 6-dione and arylidene-arylamines various 6-aryl-substituted-4-benzoylacetyl-4-hydroxy-5, 6-dihydro-4H-1, 3-oxazines were synthesized. Due to the relevance of search of new biologically active compounds with antioxidant activity, there was analyzed antiradical activity of derived oxazines. Antiradical properties of these compounds were studied through the binding reaction of stable free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH). Trolox was used as a standard. Six compounds with moderate antioxidant activity were detected. Antioxidant activity was studied on the Escherichia coli BW 25113 strain grown aerobically. When constructing a model of oxidative stress with solution of hydrogen peroxide on cells of Escherichia coli BW 25113 strain there was observed specific growth of cells per 1 hour with 6 substances of oxazine group. Two compounds have pro-oxidant activity and one compound demonstrated antioxidant activity. The outcomes of spectrophotometry with DPPH and on cells of Escherichia coli BW 25113 experiment are well correlated.